Pyridopyrimidines are a versatile class of fused nitrogen containing heterocyclic compounds that exhibit a broad spectrum of biological activities, making them a focus of extensive pharmacological research. These molecules consists of pyridine ring fused with a pyrimidine ring, forming a rigid structure that can interact with various biologic targets. The ongoing structural modifications and synthesis of novel pyridopyrimidine analogues continue to expand their scope making them promising scaffolds and also recent advancements in its synthetic methodologies have facilitated the design of structurally diverse pyridopyrimidine derivatives. This study reviews the synthesis of pyridopyrimidine along with various biological activities of pyridopyrimidines, including their roles as anti cancer, anti histaminic, antimicrobial, antiviral, and analgesic agents. Beginning with an introduction to the structural diversity and synthetic strategies for pyridopyrimidine derivatives , the review highlights their broad spectrum of pharmacological effects