Fig. 1. Phylogenetic tree of Neocaridina species based on the mtDNA COI region (644 bp) and comparison of species recognition in Shih et al. (2024) and Onuki and Fuke (2022). As a dataset, all Neocaridina spp. available in the International Nucleotide Sequence Database were downloaded and aligned using MAFFT (Katoh and Standley 2013) after removing identical sequences using vsearch 2.23.0 (Rognes et al. 2016). Phylogenetic tree estimation was based on the maximum likelihood method using IQ-TREE 2.2.2.6 (Minh et al. 2020) with the codon partitioning model selected by ModelFinder based on BIC (Kalyaanamoorthy et al. 2017). The reliability of each branch was assessed using the Ultrafast bootstrap method (Hoang et al. 2018).

Published as part of Fuke, Yusuke, 2024, Commentary: Integrative Taxonomy Reveals Freshwater Shrimp Diversity (Decapoda: Atyidae: Neocaridina) from Kyushu and Southern Honshu of Japan, with a Discussion on Introduced Species, pp. 1-4 in Zoological Studies 63 (53) on page 2, DOI: 10.6620/ZS.2024.63-53, http://zenodo.org/record/14699995