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ZENODO
Article . 2015
License: CC 0
Data sources: Datacite
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ZENODO
Article . 2015
License: CC 0
Data sources: ZENODO
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Peptide Nucleic Acid (Pna) Fluorescent In Situ Hybridization (Fish) On Chromosomes In Suspension For Analysis Of Repetitive Dna By Flow Cytometry

descriptionPublicationkeyboard_double_arrow_right Article 26 Jan 2015Publisher:Zenodo
Authors: sprotocols;

doi: 10.5281/zenodo.14571

Peptide Nucleic Acid (Pna) Fluorescent In Situ Hybridization (Fish) On Chromosomes In Suspension For Analysis Of Repetitive Dna By Flow Cytometry

Abstract

This protocol describes a method developed in our laboratory to analyze repetitive DNA in chromosomes using fluorescence in situ hybridization (FISH) and flow cytometry (chromosome flow FISH or CFF). Our protocol includes details on procedures to isolate mitotic chromosomes from adherent cell cultures, FISH using peptide nucleic acid (PNA) probes on chromosomes in suspension and flow cytometry setup and data acquisition.

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selected citations
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This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
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