The fight against resistant microorganisms has recently become increasingly important. The majority of antimicrobial drugs, including antiseptics, have successfully developed multidrug resistance in many microorganisms and viruses. Among the drugs to which the microorganisms have not yet developed resistance remain silver preparations. In this review, we offer a summary of research on the silver preparations effect not only in microorganisms, but also in the human body, including the molecular-biological aspects of such effect. The article presents results of studies silver ionic forms, colloidal and metallic form influence on microorganisms, viruses and cancer cells. The effect of various silver preparations on the biochemical pathways in the human body is also shown.