The archive contains a dataset that can be used for fish detection and size estimation. The footage was collected during spring and late summer periods from two separate sites: Bragado, located in Avelames River, and Covas Do Barroso in Couto River, both tributaries of the Tamega River (Douro River basin), Portugal. The recordings feature three species: the Northern Iberian chub (Squalius carolitertii), the Northern straight-mouth nase (Pseudochondrostoma duriense), and the brown trout (Salmo trutta). The dataset contains 18523 manually annotated multi-modal frames, encompassing both infrared (IR) and RGB modalities.