Background:In Pakistan, the blood donation and transfusion practices are based on primitive practice of replacement donors whereas all around the world, the practice of voluntary blood donation has replaced the replacement donation. The survey regarding misconceptions and motivation may help the blood donation centers to develop their future policies to inspire people for donating blood on a regular basis and to inspire non-donors to start donating blood. Objectives: To assess the knowledge, attitude and practice towards blood donation. To identify factors behind motivation for voluntary blood donation and to determine misconceptions and myths associated with blood donations Methodology: This was a descriptive cross sectional survey conducted in Islamabad. The data was collected from 204 respondents residing in urban, rural and slum areas through a questionnaire and processed in SPSS software version 20. Results:In our study 78 respondents were residing in urban areas, 51 in rural and 75 in slum areas. Out of 204 sample, only 77 were blood donors and amongst them only 29% had donated blood to unknown persons. Among 111 respondents who required blood, only7% received it from voluntary donors. The most important motivational factor for donating or intend to donate blood was helping family or friends in need (76.5%) followed by spiritual satisfaction (71.2%). Among non-donors, 52.7% had never donated blood because no one ever asked while families of 48.8% didn’t allow donation. 48% feared that it may lead to permanent weakness or anemia while 44.8 % were concerned about the sterility of equipment. Conclusion:The practice of voluntary blood donation is almost nonexistent in our population. To overcome the prevailing misconceptions and reported fears, it is important to provide adequate information about donation to potential donors. Appropriate motivational campaigns should be launched among the population to make it a regular practice in order to increase voluntary blood donation Key words: Voluntary, Donation, Motivation, Misconception