The concentration-dependent effects of gibberellic acid (GA3) and kinetin (KIN) on in vitro seed germination, organ development, and phenolic substance contents in arugula (Eruca vesicaria subsp. sativa) were investigated. The seed germination ratio and leaf production increased to 100% and 3.93 leaves, respectively, after GA3 treatment at 0.25 mg L-1. GA3 at 0.25 mg L-1 treatment also resulted in more elongated roots (4.03 cm) than the other treatments. However, the stimulative effect of GA3 on root elongation was not recorded in root production. The highest root number (1.93 roots) was recorded from the control seedlings, and KIN treatments resulted in a concentration-dependent decrease in the parameter. Hypocotyl elongation was triggered after 0.25 mg L-1 GA3 treatment. Total phenolic substance (TPC) and flavonoid content (TFC) were estimated from methanolic extracts obtained through ultrasonic extraction and expressed as gallic acid (GA) and quercetin (Q) equivalents (E), respectively. A slight increase in the TPC to 11.57 mg GAE g-1 DW was found after KIN treatment at 0.25 mg L-1 compared to the control (10.35 mg GAE g-1 DW). A similar trend was also recorded in TFCs. KIN treatments at 0.25 and 1.0 mg L-1 increased the content (7.42 and 7.40 mg QE g-1 DW, respectively) compared to the control (6.43 mg QE g-1 DW). The results suggested that GA3 and KIN treatments at 0.25 mg L-1 can enhance arugula's growth parameters and phenolic substance content, respectively.

Kocaeli Science Congress 2024 (KOSC-2024), 02-04 October 2024, Kocaeli / TÜRKİYE https://fefkongre.kocaeli.edu.tr/en