The software FishInspector allows annotation of features in images of zebrafish. The recent version requires images of a lateral position. It is important that the position is precise since deviation may confound with feature annotations. Images from any source can be used. However, depending on the image properties parameters may have to be adjusted. Furthermore, images obtained with normal microscope and not using an automated position system with embryos in glass capillary require conversion using a KNIME workflow (the workflow is provided as well). As a result of the analysis the softare provides JSON files that contain the coordinates of the features. Coordinates are provided for eye size, fish contour, notochord curvature, otolith eye distance, yolk sac, pericard and swimbladder. Furthermore, the intensity of pigmentation is calculated for the notochord area. Additional features can be manually annotated. It is the aim of the software to provide the coordinates, which may then be analysed subsequently to identify and quantify changes in the morphology of zebrafish embryos.

The software was developed as part of the project ZFminus1 funded by the German Ministery of Education and Resarch (BMBF). Grant identifier 031A582.