This dataset contains the Underlying Data to the paper “ Dimensions, stability and deformability of DOPC-cholesterol Giant Unilamellar Vesicles formed by droplet transfer”. “Manifest.toml” Julia computational environment file “Project.toml” Julia computational environment file “_init.jl” script initialising the Julia computational environment based on the “Manifest.toml” and “Project.toml” files “deformability.jl” script to analyse the magnetic deformation of ferrofluid-loaded GUVs with and without cholesterol “size_distribution_stability.jl” script to analyse the size distribution and overnight stability of GUVs with different DOPC:Chol ratios “data” folder containing the datasets with the sizes of all the observed GUVs GUVs_stability_ON GUVs_stability_T0 GUVs_with_cholesterol_6040_1 GUVs_with_cholesterol_6040_2 GUVs_with_cholesterol_6040_3 GUVs_without_cholesterol_1 GUVs_without_cholesterol_2 GUVs_without_cholesterol_3 “utilities” folder containing the script to calculate the magnetic field generated by our permanent magnets device “magnetic_fields.jl”