Abstract The study on the Sacred water in Osun Osogbo, aimed to unravel it sacred waters cape through a multidimensional phases. .This study employs oral interviews and leverages secondary sources as methodological tools to delve into the archaeological facet, the ancient artifacts, ritual objects, woodcarving, pottery, and remnants of Osun Osogbo Sacred Grove. The study will examine its pilgrimage traditions, during the annual pilgrimage traditions, devotees converge in the town of Osun, typically in August each year, to celebrate and partake in the sacred rituals of Osun Osogbo, the study traverse the sacred topographies and associated saint cults known as Yeye Osun "Osun's Mother" and the Arugba known as the "Bearers of the calabash."The study will also examine the profound traditions rooted in the belief that the deity, Osun, grants requests and abundantly blesses worshippers .Recognized as the Ile-Aje" House of Riches, “how Osun Osogbo holds a pivotal role in fulfilling desires, including the blessing of fertility, granting children to barren women. Findings showcase the unique archaeological characteristics that define Osun Osogbo, while therapeutic landscapes interweave with the intricate of medical humanities surrounding healing waters. Beyond its physicality, Osun Osogbo emerges as an exus of social relations between humans and non-human entities. As an ecosystem, it harmoniously incorporates associated trees, rocks, and sacred topographies. This study aim to contribute to a holistic understanding of Osun Osogbo, providing insights into its archaeological roots, therapeutic significance, cultural histories, and the interconnected web of human-nature relationships. Keywords: Sacred, Tradition Biocultural, cultural, Festival Grove