The study was to assess the knowledge regarding prevention of hepatitis B among the IVth class workers of selected urban heath centers of Sangli, Miraj and Kupwad corporation area. The quantitative research approach was used. Descriptive survey design was used for the study. The study was conducted in urban heath centers of Sangli, Miraj, Kupwad corporation area. The non probability convenient sampling technique was used and study consisted of 50 samples as per criteria. Based on objective the structured questionnaire was used for collecting data. The tool was divided into 2 sections the section-I contains demographic variables for the IV class workers and section ?II contains 21 multiple choice questions with 4 options for assessing the knowledge. Validity of tool was done by 15 experts.. Karl pearson correlation coefficient and Browns prophecy formula were used for the estimation of reliability. The reliability coefficient ?r? of the questionnaire was 0.94 which is more that 0.7 hence it is found to be reliable. 6 samples were taken for pilot study. The findings concluded that 90% 4th class workers had average knowledge regarding prevention of hepatitis B and the knowledge developed by researcher found to be enhancing the knowledge of IV class workers regarding prevention of hepatitis B .Study concluded that there is need for teaching to class IV worker of health settings regarding prevention of occupational hazards