This image illustrates cpYFP-reported mitoflashes in isolated heart mitochondria supported by 3 mM ATP, extracted from a video within the research on mitoflash biogenesis. The mitoflash events are captured under conditions where Complex V operates as an ATPase. Scale bar, 2 µm. Source: The original video and data are part of the study: "Mitoflash biogenesis and its role in the autoregulation of mitochondrial proton electrochemical potential," published in Journal of General Physiology on March 15, 2019. For more details, refer to the original article by Gaomin Feng, Beibei Liu, Jinghang Li, Tianlei Cheng, Zhanglong Huang, Xianhua Wang, and Heping (Peace) Cheng. Citation: Feng, G., Liu, B., Li, J., Cheng, T., Huang, Z., Wang, X., & Cheng, H. (2019). Mitoflash biogenesis and its role in the autoregulation of mitochondrial proton electrochemical potential. Journal of General Physiology, 151(6), 727–737. DOI: 10.1085/jgp.201812176