QUAST stands for QUality ASsessment Tool. It evaluates genome/metagenome assemblies by computing various quality metrics (more than 50 in the current release). The current QUAST toolkit includes the general QUAST tool for genome assemblies, MetaQUAST, the extension for metagenomic datasets, QUAST-LG, the extension for large genomes (e.g., mammalians), and Icarus, the interactive visualizer for these tools. The QUAST package works with and without reference genomes. However, it is much more informative if at least a close reference genome is provided along with the assemblies. The tool accepts multiple assemblies, making it suitable for comparison.