Data from " Allostery can convert binding free energies into concerted domain motions in enzymes" Electrophysiology data corresponding to the main text figures and supporting information figures. One representative set was chosen for each triplicate and included in this data set. For details are found in the ‘read me explanation.txt’ PDB used for this paper can be found at; 4ake [http://doi.org/10.2210/pdb4AKE/pdb] and 1ake [http://doi.org/10.2210/pdb1AKE/pdb] Full uncropped scans of any cropped gel/blot images are provided. The zip folder contains the MATLAB code package HMM inference, specifically tailored to nanopore ionic current flow data, as analyzed in the publication and can also be found at: https://github.com/yulanvanoppen/nanopore-HMM