Table 2 Enhancement of somatic embryos and germination frequency in cell suspension culture from friable embryogenic callus (FEC) of L. montana. Sucrose (g L − 1) + PGR (μM) in solid MS mediumPGR (μM) in liquid MS mediumLiquid MS medium (MSL)Number of SEs/SCV developmental stageGermination (%)6 weeks of culture4 weeks of culture2 weeks of cultureGlobularDifferent stages of embryo*35 + 10 picloram + 2 BA1 picloramMSL10.0 e6.4 e01 2,4-DMSL14.4 cd9.0 cd01 2,4-D + 0.5 BAMSL15.0 cd9.8 cd7.2 e1 2,4-D + 0.5 m TRMSL7.8 f6.0 e8.6 de1 2,4-D + 0.5 TDZMSL18.0 b12.4 b14.0 ab35 + 10 2,4-D + 2 TDZ1 picloramMSL6.8 fg3.0 f01 2,4-DMSL16.0 cd6.8 e01 2,4-D + 0.5 BAMSL18.4 b10.0 c11.4 c1 2,4-D + 0.5 m TRMSL10.2 e6.8 e14.3 ab1 2,4-D + 0.5 TDZMSL21.4 a14.6 a15.2 a PGR = Plant growth regulator. MSL = Liquid MS medium.SEs = Somatic embryos.SCV = Settled cell volume.*Different stages of embryo = pear-shaped, early torpedo-shaped, torpedoshaped and cotyledonary-stage embryos. Values with the means derived from 5 replicate (each 500 mg FEC) with 1000 μL SCV of embryogenic suspension cells per replicate. Mean values followed by same letters in each column are not significantly different according to the Duncan's multiple range test at 5% level.

Published as part of Baskaran, P. & Van Staden, J., 2017, Ultrastructure of somatic embryo development and plant propagation for Lachenalia montana, pp. 270 in South African Journal of Botany 109 (10) on page 270, DOI: 10.1016/j.sajb.2017.01.006, http://zenodo.org/record/14036627