Effective infection prevention and control (IPC) is essential to ensure high-quality healthcare services. Infections that occur during hospitalization and because of services provided may interfere with the outcome of needed medical treatments. To prevent healthcare-associated infections (HAIs), it is essential that all healthcare personnel are well trained in and follow standard IPC measures during patient care. Standard precautions include hand hygiene, use of personal protective equipment and more. The Norwegian Institute of Public Health (NIPH) is introducing a new electronic tool and national template for direct observation of compliance with recommended IPC measures in healthcare. The solution is called the National Tool for Observation of Infection Prevention Measures (NOST). NOST is a quality improvement tool that includes a web-based solution for observing and recording the degree of compliance with recommendations for hand hygiene and other IPC measures. The trial protocol is available at https://zenodo.org/records/7648821