In the present research work the phytochemical investigation of methanolic, ethanolic and chloroform extracts ofBerberis lycium and pharmacological activities of methanolic extracts (Anti-inflammatory and analgesic activity) and antibacterialactivities in methanolic, ethanolic and chloroform extracts was carried out. The phytochemicals analysis showing the presence ofcarbohydrates, flavonoids, phlobatannins, alkaloids, saponins, tannins, phenols, terpenoids, cardiac glycosides was present in methanolic and ethanolic extracts, while alkaloids, phlobatannins, glycosides and protein were absent and quantative phytochemistry showed the flavonoids in chloroform extract as (14.20?0.15mg/ml), Alkaloids (12.10?0.15mg/ml), phenolics (10.45? 0.10mg/ml), Saponins (06.22?0.14mg/ml) and Tannins (04.60?0.65 mg/ml). The pharmacological activities such as Anti-inflammatory, at the doses of 600 mg/kg b.w the administration of extract produced a significant anti-inflammatory activity at 3 hours with paw oedema inhibition of 59 % respectively, while the standard drug aspirin inhibited paw oedema of 68%. In analgesic activity the results showed significant dose dependent % inhibition of pain responses in extract 600mg/Kg is 61%. In antibacterial activity the most active among the extracts was with (17.00?0.48 mm) zone of inhibition at the concentration of18 mg/?l against Pseudomonas aeroginosa. Fallowed by Escherchia coli(16.27?0.93mm), Shigella flexneri(16.20?1.89mm) and Salmonella typhi(16.11? 0.82) with concentration of 12 mg/?l.