Dataset contains Drosophila melanogaster behavioral annotations of chasing. The dataset is formed by 20 GAL4 line flies group from a TrpA activation screen with increased propensity to chasing and by 19 pBDPGAL4U line (control) flies group. Ctrax motor trajectories derived from the original video-recordings (1000 seconds) were downloaded from this link and used to obtain the chasing behavioral annotations using JAABA 10.1038/nmeth.2281: The data set consist in: - a "mappings" folder containing all the mappings used by the pergola in the pipeline to convert data. - a "perframe_TrpA" folder containing mat files with three Ctrax derived variables (dnose2ell, dtheta and velmag) from the motor trajectories of the GAL4 line. - a "perframe_pBDPGAL4" folder containing mat files with three Ctrax derived variables (dnose2ell, dtheta and velmag) from the motor trajectories of the control line. - a "scores" folder including the JAABA chasing annotations in two mat file one for each fly line.