Live imaging microscopy data set used in the publication "Coordination between endoderm progression and mouse gastruloid elongation controls endodermal morphotype choice". Farag et. al., Dev Cell 2024 Sep 9;59(17):2364-2374.e4. doi: 10.1016/j.devcel.2024.05.017. The data set contains: Epifluorescence time series imaging of Brachyury-GFP/Sox17-RFP gastruloids between 48 and 96 hours post aggregation. Format : .nd2 files (can be opened with NIS Viewer or Fiji software). Excel table with raw and reduced set of morphological and expression features manually extracted from this data set. The reduced set (DT) was used in our paper to learn the decision tree classifiers.