ABSTRACT According to a 2020 survey, 45% of the Indian population utilizes spectacles. The aim of this study is to identify and extract DNA from the nose pads of bespectacled individuals, which can serve forensically in the identification and resolution of crime cases. It has been observed that sweat accumulated around the nose pads of bespectacled individuals can potentially contain DNA for individualization. The presence of DNA in human sweat suggests the presence of cells, such as sloughed-off skin cells, within the sweat itself. In this study, Sweat-derived DNA was separated and quantified using agarose gel electrophoresis (0.8%). After that, 1.0ng of the DNA was amplified by multiplex PCR using PowerPlex 21 and GlobalFilerTM kits. Using genetics analyzers 3130 and 3500XL, capillary electrophoresis of the amplified products was carried out. Following that, the data were examined using GeneMapper ID Software Version 3.2 and GeneMapperTM ID-X software v1.6, which yielding successful identification results. Despite varying weather conditions, complete DNA profiles were obtained from 87.3% of the samples. The findings suggest that sweat collected from bespectacled individuals can serve as a reliable source for DNA profiling, offering potential advancements in forensic investigations.