This study introduces Stratya2D, a novel Python-based program that significantly advances basin analysis by enhancing the accessibility of decompaction and backstripping processes. Stratya2D employs a novel approach that streamlines the integration of seismic horizons with decompaction and backstripping analyses, thereby providing enhanced 2D visualisation capabilities. The program not only calculates burial thickness and tectonic subsidence but also integrates these measurements with horizons extracted directly from seismic images provided by the user. Utilising advanced image processing techniques, Stratya2D facilitates the direct extraction of geological horizons from simple seismic images, marking a breakthrough in geoscientific analysis. Our methodology transcends traditional 1D approaches, which typically represent results as discrete points across timelines, by using 2D seismic cross-sections for comprehensive basin-wide analysis. This approach offers a broader spatial perspective and simplifies complex geological assessments through the integration of well data, thereby enhancing our understanding of basin geology. The analytical capabilities of Stratya2D, accessible even to users with basic Python skills, reduce the need for costly and specialised software. The program includes an error quantification method employing Monte Carlo simulation to refine the analysis of depositional thickness and tectonic subsidence curves. The effectiveness of Stratya2D was demonstrated through a comparative analysis with established literature and specific case studies, including the recent exploration of the NDI Carrara 1 well in the South Nicholson Region of the Northern Territory, Australia. The results not only align closely with previously published data, accurately replicating the tectonic subsidence curve but also extend these findings to the complex South Nicholson Region. The consistency of our results with those obtained from industry-standard PetroMod software highlights the reliability and precision of Stratya2D in conducting geological assessments. This program offers a user-friendly, economical tool for basin analysis, delivering detailed insights into the geological evolution on a 2D scale and surpassing traditional 1D perspectives.