The study focused on the fish seed marketing network at Digharkanda, near BFRI, aiming to identify the diversity of actors involved, distribution patterns of fish seed across Bangladesh, seasonal demand, and value chain benefits for various stakeholders. Primary data were gathered from Fish Seed Selling Shops (FSSSs) during the breeding season. Results showed that 65% of fish seed was sold locally within Mymensingh, while 35% was distributed to other districts. Each FSSS employed 8-9 individuals, sustaining livelihoods through the fish seed business. During the breeding season (March to October), FSSSs transacted approximately 2524 kg of hatchery-produced seed, 2000 kg of nursery-produced seed, and 2500 kg from external sources. The marketing channel generated profit margins of BDT 3,350,000 for hatcheries and BDT 6,000,000 for nurseries. Fish seed demand was found to fluctuate seasonally, but the year-round marketing of catfish and carp seeds helped sustain FSSS operations. Notably, Chital seeds were priced higher than others, with tilapia seeds being the lowest in price. The study also identified several key challenges faced by seed producers and traders, including brood fish disease, inbreeding issues, transportation difficulties, and limited access to credit.