AudioSpike is a free open source software package for electrophysiological measurements using acoustical stimulation for Microsoft® Windows®. Instead of using proprietary hardware to record electrode signals AudioSpike uses standard sound cards for acoustcial stimulation and recording electrode signals. The recorded spikes can be evaluated using multichannel cluster analysis and PSTH selection in realtime. Interfaces from/to MATLAB® and GNU Octave respectively for setting up the measurement tasks and reading raw measurement data and evaluation data back into the workspace are shipped with AudioSpike. Take advantage of multichannel spike detection (recording) multichannel acoustical stimulation real-time GUI interfaces for spike selection, cluster analysis, PSTH, vector strength and much more Special search modes with realtime spiketrain and spike visualization calibration and frequency equalisation interfaces in-situ calibration procedure no proprietary hardware required: use standard sound cards for spike recording interfaces from/to MATLAB®/Octave support for offline analysis: re-analyze spike trains after setting new detection thresholds, cluster selections ... More details, version history and binary installer files see https://www.audiospike.de