Image Metadata (using REMBI template): Study Study description Creation of a beautiful image for an image exhibition General dataset info Christian Jüngst (Acquisition), Working group of Prof. Matteo Bergami (Sample Preparation) Study Component Imaging method Multiphoton microscopy Biosample Biological entity Nerve cells Organism Mus musculus Organism part Dentate gyrus of the hippocampus Specimen Preparation method Tissue section of a mouse brain Signal mechanism Fluorescent proteins Channel 1 - content EYFP Channel 1 - biological entity Thy1 Image Acquisition Instrument attributes TCS MP-OPO, Leica Microsystems. Upright Multiphoton microscope with a 25X water immersion objective Image acquisition parameters Acquisition of a z-stack with 173 steps in an interval of 2 µm Image Data Type Maximum projection with color coded depth Dimension extents x: 1024, y: 1024, z:173 Size description 442.86 µm x442.86 µm x 343.79 µm Pixel size description 0.433 µm x 0.433 µm x 2 µm Image processing method A Median filter was used to smooth the image and pixel amount was increased to print it out for the image exhibition

Raw microscopy image from the NFDI4Bioimage calendar October 2024.