Every plant species created by nature has more or less medicinal properties. Medicinal plants have been used in Indian culture since the dawn of human civilization. Plants are found to be directly and indirectly involving in treatment of human ailments and improve socioeconomic status. Traditional healers of Northeast India have gained a special reputation in treating various terminal diseases. This research was carried out in Tripura state to preserve the traditional medicinal knowledge of the plants. Traditional healers were chosen through field survey, and ethnomedicinal data were collected using semi-structured, and open-ended interviews. Information on traditional practitioners age, place of practice, experience, learning methods, diseases treated, diagnostic approaches, as well as the storage and usage of plants, were systematically gathered during the field interviews. A total of one hundred respondents have provided data, the majority of whom were from the Scheduled Tribe category and had a middle socioeconomic background. The results of the Pearson's Correlation test indicate a significant association between caste, age, and gender with ethnobotanical knowledge at 0.05 level. Traditional healers utilize 248 plant species belonging to 56 genera and 37 families which treat 75 types of ailments. Analysis of ethnobotanical indices such as UR, UV, FC, RFC, and FL (%) values indicate that Tinospora cordifolia, Piper nigrum, Zingiber officinale and Antidesma roxburghii were found to be most potential plant species against certain specific ailments according to practitioner knowledge. The fact that hepatobiliary disease has the highest ICF value (0.37) suggests that these conditions are prevalent in the research area and that the species has traditionally been utilized to treat them. Phytoconstituents and pharmacological activities are unquestionably required to validate the ethnic uses of potential plants. Everyone should participate in the preservation of floral diversity since plants are essential for the production of new drugs for preventing a variety of complicated disorders.