A reverse phase high performance liquid chromatography method was developed for the simultaneous estimation of diloxanide furoate and metronidazole in formulation. The separation was achieved by octadecyl C8 column and a mixture of methanol: acetonitrite: 0.05M phosphate buffer at pH 4.0 (45:25:30 v/v) as eluent, at a flow rate of 1 ml/min. detection was carried out at 277 nm. Quantitation was done by external standard method. The retention time of metronidazole and diloxanide furoate was found to be 3.28 and 6.42 min, respectively. The method has validated for linearity, accuracy and precision. Linearity of metronidazole and diloxanide furoate were in the range of 5-50 µg/ml for both the drugs The mean recoveries obtained for metronidazole and diloxanide furoate were100.01% and 99.71%, respectively. The developed method was found to be accurate, precise, selective and rapid for the simultaneous estimation of metronidazole and diloxanide furoate in tablet. Key- words: RP-HPLC, metronidazole, diloxanide furoate, simultaneous estimation