Levamisole was originally used as an anthelmintic to treat worm infestations in both humans and animals. Two simple UV spectrophotometric methods have developed for determination of levamisole in bulk and formulations. The absorption maxima (λmax) of levamisole was found to be 216nm in 0.1 N HCl and in double distilled water observed at 215 nm and Beer-Lambert’s law was obeyed over the concentration range 2-10µg/ml.LOD and LOQ values of levamisole was found to be 0.125 µg/ml, 0.416 µg/ml and 0.098 µg/ml, 0.326µg/ml in 0.1 N HCl and double distilled water respectively. The validation parameters were treated statistically with ‘t’ test and significant differences were observed. The study clearly revealed that the solvents influence the determination of levamisole. The methods developed were rapid and easily can be applied for the estimation of levamisole in bulk and pharmaceutical formulations.