Reference datasets (Dec 2024 update) for Mitohelper (https://github.com/aomlomics/mitohelper) Mitohelper is a repository built to facilitate experimental design, alignment visualization, and reference sequence analysis in fish eDNA studies. Refer to our paper and Mitohelper's wiki for database construction pipeline. I. Reference database files in tab-separated format, containing gene, taxonomy, and sequence information: mitofish.all.Dec2024.tsv (874,975 records) mitofish.12S.Dec2024.tsv (60,610 records of 12S rRNA gene >50 bp long) mitofish.12S.Dec2024_NR.fasta (FASTA file of 12S rRNA gene records) mitofish.COI.Dec2024.tsv (318,682 records) II. De-replicated QIIME 2-compatible 12S/12S+16S+18S rRNA reference datasets: 12S-seqs-derep-uniq.qza 12S-tax-derep-uniq.qza 12S-16S-18S-seqs.qza 12S-16S-18S-tax.qza If you use Mitohelper, please cite:Jean Lim, S, Thompson, LR. Mitohelper: A mitochondrial reference sequence analysis tool for fish eDNA studies. Environmental DNA. 2021; 00: 1– 10. https://doi.org/10.1002/edn3.187