FIGURE 9. Field-collected specimens of Cladophoraceae sp. 3 (Rhizoclonium umbraticum). (A) Morphologies of field-collected specimens. (B, C) Filamentous thalli with non-septate rhizoids. (D–F) Spindle- and band-shaped chloroplasts with pyrenoids (arrow heads). (G, H) Autofluorescence of chloroplasts. (I) Cells stained with DAPI, showing nuclei. (J) Cells stained with Lugol's iodine, showing pyrenoids. (K, L) Pyrenoids observed by transmission electron microscopy. Pyrenoids with starch plates. (K) A thylakoid membrane; (L) Three thylakoid membranes traversing a pyrenoid. Scale bars = 80 μm (A–C); Scale bars = 10 μm (D–H); Scale bars = 40 μm (H, J); Scale bars = 0.5 μm (K, L).

Published as part of Ichihara, Kensuke, Shimada, Satoshi & Miyaji, Kazuyuki, 2016, Two new green algae, Rhizoclonium fractum sp. nov. and R. umbraticum sp. nov., from tropical and subtropical brackish waters of Japan, pp. 231-249 in Phytotaxa 266 (4) on page 244, DOI: 10.11646/phytotaxa.266.4.1, http://zenodo.org/record/13658104