FIGURE 8. Secondary structures for the BoxB and V3 helices in the ITS regions for Roholtiella spp. and representative outgroup taxa, C. cimrmanii and N. indistinguendum. Arrows and a bold font style show bases variable among species. Helices are arranged by taxon in vertical columns, with taxon label next to the V3 helix. A–F. BoxB helices from operons with both tRNA genes. A. Roholtiella edaphica strains CCALA 1055-56, CCALA 1060-61. B. Roholtiella edaphica strain CCALA 1062. C. Roholtiella mojaviensis strains CCALA 1051- 52. D. Roholtiella bashkiriorum strains CCALA 1057 and 1059. E. Roholtiella fluviatilis strain CCALA 1058. F. Nostoc indistinguendum strain CM1-VF10. G–M. BoxB helices from operons with no tRNA genes. G. Roholtiella edaphica strains CCALA 1055 and 1060. H. Roholtiella edaphica strain CCALA 1062. I. Roholtiella mojaviensis strain CCALA 1052. J. Roholtiella bashkiriorum strain CCALA 1057. K. Roholtiella fluviatilis strain CCALA 1058. L. C. cimrmanii strain CCALA 1012. M. Nostoc indistinguendum strain CM1-VF10. N–T. V3 helices. Circled residues represent alternate bases in different strains, or in the case of R. fluviatilis, between different operons in the same strain. N. The structure without the circled bases was obtained for R. edaphica strains CCALA 1060 (no and both tRNA operons) and CCALA 1056 (no tRNA operon).The alternative structure with the circled bases was obtained for strains CCALA 1061 (no tRNA operon) and CCALA 1055 (no and both tRNA operons). O. Roholtiella edaphica strain CCALA 1062. P. Roholtiella mojaviensis strains CCALA 1051-52. Q. The structure without the circled bases was obtained for R. bashkiriorum strains CCALA 1057 (both tRNA operons) and CCALA 1059 (no tRNA operon). The alternative structure with the circled bases was obtained for strain CCALA 1057 (no tRNA operon). R. The structure without the circled bases was obtained for R. fluviatilis strain CCALA 1058 for the operon with both tRNA. The alternative structure with the circled bases was obtained for the operon with no tRNA. S. C. cimrmanii strain CCALA 1012 showing the

Published as part of Bohunická, Markéta, Pietrasiak, Nicole, Johansen, Jeffrey R., Gómez, Esther Berrendero, Hauer, Tomáš, Gaysina, Lira A. & Lukešová, Alena, 2015, Roholtiella, gen. nov. (Nostocales, Cyanobacteria)-a tapering and branching cyanobacteria of the family Nostocaceae, pp. 84-103 in Phytotaxa 197 (2) on page 99, DOI: 10.11646/phytotaxa.197.2.2, http://zenodo.org/record/13639648