FIGURE 7. Secondary structures for the D1–D1' helix in the ITS regions for Roholtiella spp. and representative outgroup taxa, Calochaete cimrmanii and Nostoc indistinguendum. Arrows and a bold font style show bases variable among species, while the circled adenine residue is an alternate base in R. edaphica CCALA 1063 and circled cytosine is an alternate base in N. indistinguendum. A–B. Equally thermodynamically stable structures in R. edaphica are shown for the following strains: CCALA 1061 for the operon with no tRNA genes; CCALA 1055-56, 1060, 1062 for the no tRNA operon as well as the operon with both tRNA genes. C–D. Differences in operons are shown for R. mojaviensis. C. Operon with no tRNA for CCALA 1051 and 1052 as well as the operon with both tRNA for CCALA 1052. D. Variation of the operon with no tRNA of CCALA 1051. E. No sequence differences existed between operons with no or with both tRNAs for R. fluviatilis (CCALA 1058), and R. bashkiriorum (CCALA 1057, 1059). F. D1–D1' helix for C. cimrmanii (strain CCALA 1012) showing the operon with no tRNA. G. D1–D1' helix for recovered operons with and without both tRNAs for N. indistinguendum (strain CM1-VF10).

Published as part of Bohunická, Markéta, Pietrasiak, Nicole, Johansen, Jeffrey R., Gómez, Esther Berrendero, Hauer, Tomáš, Gaysina, Lira A. & Lukešová, Alena, 2015, Roholtiella, gen. nov. (Nostocales, Cyanobacteria)-a tapering and branching cyanobacteria of the family Nostocaceae, pp. 84-103 in Phytotaxa 197 (2) on page 98, DOI: 10.11646/phytotaxa.197.2.2, http://zenodo.org/record/13639648