Abstract The high use of mosquito repellent has become the norm in preventing the spread of diseases caused by mosquitoes. Meanwhile, mosquito spray contains active ingredients such as Endocrine Disruptive Chemicals. This chemical has an impact on the stimulation of FSH and LH excretion and has a direct effect on the disruption of follicular development (folliculogenesis) in the ovary. Rice bran oil (RBO) has potential as a folliculogenesis treatment as it contains antioxidants that can reduce the effects of damage to plasma lipids in mitochondria and can block chronic inflammation. Aims: To analyze the effect of subacute administration of RBO (for 28 days) as an anti-inflammatory in controlling folliculogenesis due to transfluthrin. Methods: Rats were divided into four groups, namely negative controls (without treatment), positive controls (groups given RBO), a group with one-push exposure, and a group with one-push and RBO. One-push exposure contains a 21.3% transfluthrin active ingredient. RBO treatment was done for 28 days. Rats were then put under an inhaled sub-chronic toxicity test for 6 hours. The samples were analyzed using a post-test only control group design, with a completely randomized design and MANOVA statistical analysis in IBM SPSS Statistics 25 software. Results: There were significant differences in ovarian weight (p = 0.022) and the number of pathological follicles (p = 0.009) in all groups. Conclusion: RBO as a source of antioxidants can reduce the number of follicular abnormalities in the ovary caused by exposure to one-push aerosols containing transfluthrin. Keywords: follicle, one-push, ovarium, rice bran oil, transfluthrin