Microspheres are ideal targeting drug delivery system with high safety profile. The objective of the present study is to reduce dosing frequency and improve patient compliance by designing and systematically evaluating mucoadhesive microspheres. Various methods have been used to solve the issue associated to barriers, such as exploring alternate methods of delivering substances. Mucoadhesion is a topic of current interest in the design of drug delivery systems. Mucoadhesive micro-spheres exhibit a prolonged residence time at the site of application or absorption and facilitate an intimate contact with the underlying absorption surface and thus contribute to improved and/or better therapeutic performance of drugs. Mucoadhesive microspheres containing itraconazole were able to enhance the bioavailability of the medicine at the oral buccal site for the treatment of oral candidiasis. The IMM5 formulation, which consists of mucoadhesive microspheres containing itraconazole, is considered the most effective formulation. It is composed of a blend of naturally occurring polysaccharides, namely Drug: HPMC: Xanthan gum in a ratio of 1:1:1. This formulation releases over 98.13% of the drug in a controlled and sustained manner for up to 12 hours in the gastric environment.