The article presents data on the study of obtaining a chemical composition from a dry extract of the medicinal plant artichoke spiny, as well as some biological active substances in the dry extract. Metrological characteristics of the results of quantitative determination of hydroxycinnamic acids have been developed. The results showed that the dry extract contained hydroxycinnamic acids such as flavonoids, tannins, and amino acids. The amount of hydroxycinnamic acids in terms of chlorogenic acid is 7.12% on average. Thus, the results obtained indicate a fairly high content of hydroxycinnamic acids in the dry extract. And the content of tannins in the dry extract averaged 6.39%. Quantitative determinations of some biologically active substances by HPLC (High Performance Liquid Chromatography (High Pressure Liquid Chromatography) in the dry extract of prickly artichoke showed a high content of chlorogenic acid, cinaroside and caffeic acid.