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Software . 2025
License: CC BY
Data sources: ZENODO
image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
ZENODO
Software . 2024
License: CC BY
Data sources: ZENODO
ZENODO
Software . 2025
License: CC BY
Data sources: Datacite
ZENODO
Software . 2025
License: CC BY
Data sources: Datacite
ZENODO
Software . 2024
License: CC BY
Data sources: Datacite
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Molar Ratio Calculator for Gibson Assembly and T4 Ligation Reactions

integration_instructionsResearch softwarekeyboard_double_arrow_right Software 10 Sep 2024Embargo end date: 10 Sep 2024 English Publisher:Zenodo
Authors: Stokes, Dallin; Lane, Kristin;

doi: 10.5281/zenodo.16884124 , 10.5281/zenodo.13621607 , 10.5281/zenodo.13621608

Molar Ratio Calculator for Gibson Assembly and T4 Ligation Reactions

Abstract

A Shiny app for calculating reaction recipes for Gibson Assembly (InFusion and NEBuilder) and T4 ligation reactions. The user inputs the total number of nanograms they are aiming for in the reaction and inputs the name, stock concentration, length, and desired molar ratio for up to six DNA segments. The app then automatically generates an optimized reaction recipe that meets the desired molar ratio and has a total DNA content as close as possible to the target number of nanograms while rounding volumes to the nearest tenth.

Related Organizations
Keywords

Molecular biology, T4 ligation, Molecular genetics, Gene Cloning, Calculator, Ligation, Gibson Assembly

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average