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ZENODO
Article . 2014
License: CC 0
Data sources: Datacite
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Article . 2014
License: CC 0
Data sources: ZENODO
https://dx.doi.org/10.5281/zen...
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https://dx.doi.org/10.5281/zen...
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Beta-Glactasidase Stain

descriptionPublicationkeyboard_double_arrow_right Article , Other literature type 30 Dec 2014Publisher:Zenodo
Authors: sprotocols;

doi: 10.5281/zenodo.13539

Beta-Glactasidase Stain

Abstract

### Solutions 1. Solution A - 5mM potassium ferricyanide, crystalline - 5mM potassium ferrocyanide, trihydrate - X-gal Stock Solution - 40mg/mL in DMSO (100mg in 2.5ml DMSO) - Final X-gal Solution - Dilute X-gal Stock Solution 1:30 in Solution A - (warm Solution A to 37°C to prevent precipitation of X-gal) ### Staining Protocol Start with fixed frozen 10μm tissue sections on pap-penned slides 1. Allow slides to completely dry for 1 hour - Fix tissue with 2% formaldehyde + 0.2% glutaraldehyde for 5 min. - Rinse with 1xPBS - Incubate slides in x-gal solution in humidity chamber at 37°C overnight - Rinse with 1xPBS - Immerse slides in 100% ethanol until all precipitate dissolves (1-3 hours) - Place slides in: - a. Eosin Y 25 seconds - b. 95% Ethanol 1 minute - c. 100% Ethanol 1 minute - d. 100% Ethanol 1 minute - e. 100% Ethanol 1 minute - f. Xylene 2 minutes - g. Xylene 10 dips - Coverslip slides using Cytoseal 60

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This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
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