Depression is considered as affective mood disorder which is characterized by change in mood, lack of confidence etc. depression is the most prevalent disorder and the symptoms associated with depression changes the neurotransmitter levels in brain such as norepinephrine, seritonine and dopamine. Tradescantia spathacea is the plant selected to be used as a test drug in experimental animals. After selecting the plant, it has been dried and powdered, which was futher extracted using methanol as a solvent in a soxhlet apparatus. The evaluation of CNS activity has been done on mice of four groups by following standard procedures with the help of experimental methods such as Muscle Relaxant Property By Rotarod Apparatus, Anti Anxiety Effect By Elevated Plus Maze Apparatus and Anti Depressant Activity By Using Actophotometer to evaluate the potency of the drug and to know its activity. After comparing the test drug (Diazepam) with standard (Plant extract), results have shown that the extract has CNS activity. Therefore, it is concluded that Tradescantia spathacea extract has strong CNS activity on mice. Key Words: seritonine, dopamine, Tradescantia spathacea, Anti-Depressant Activity, and standard drug (Diazepam).