The present study was designed to investigate the effects of aqueous extract of Indian costus roots on lipid profile in a diet-hypercholesterolemic mice. The subjects were randomized into 3 groups: Group A (Normal Control); Group B ( Hypercholesterolemic Control); Group C (Hypercholesterolemic + Indian costus treated). Hyperlipidemia was induced by feeding high fat diet. After 3 weeks of hypercholesterolemic diet , body weights, blood cholesterol level, triglycerides , Plasma enzyme ALT, AST and ALP levels were measured. The oral administration of aqueous extract of Indian costus roots shows reduction in total cholesterol, triglycerides and Plasma enzyme ALT, AST and ALP levels in hypercholesterolemic mic compared to normal group. The findings of this study indicated that the administration of Indian costus improved level of lipid profile and decreased in the Plasma enzyme ALT, AST and ALP levels in hypercholesterolemic mice.