This report presents a comprehensive biotechnical analysis of fillets and byproducts (heads, bones/frames, and skin) from Cisco (Coregonus Artedi) harvested in the Great Lakes region of the United States. Conducted by Matís for the Conference of Great Lakes St. Lawrence Governors & Premiers (GSGP), the study aims to identify higher-value alternative uses for Cisco byproducts. Key findings include: Whole Body Mass Balance and Proximate Composition: Detailed analysis of fillets, heads, bones, and skin. Amino Acid and Hydroxyproline Content: Evaluation of amino acid content in heads and hydroxyproline content in skins and scales, with scales showing notably high hydroxyproline at 2.45 g/100g protein. Utilization Alternatives Protein Isolate and Fish Oil from Heads: The heads showed significant amino acid content, making them suitable for protein isolate production and fish oil extraction. Gelatine Production from Skins and Scales: High hydroxyproline levels indicate potential for high-quality gelatine production. Protein Hydrolysates from Viscera and Roes: Though promising, these transformations require substantial investment and adequate raw material supply. Conclusion and Recommendations This initial identification of utilization alternatives highlights the potential for value-added products from Cisco byproducts. Further analysis and feasibility studies are recommended to optimize and implement these alternatives.