When conserving or protecting rare or endangered species, current general guidelines for reducing light pollution might not suffice to ensure long-term threatened species’ survival. Many protected areas are exposed to artificial light at levels with the potential to induce ecological impacts with unknown implications for the ecosystems they are designated to protect. Consequently, it is recommended that precautionary methods for the avoidance and mitigation of light pollution in protected areas be integrated into their management plans. This paper’s aims are to present an overview of best practices in precautionary methods to avoid and mitigate light pollution in protected areas and to identify and discuss what ecosystems should be considered light-sensitive and how to prioritise species and habitats that need protection from artificial light, including examples of legislation covering ecological light pollution in the European Union and in Sweden. The important aspects to include when considering light pollution at a landscape level are listed, and a proposal for prioritisation among species and habitats is suggested. Sensitive and conservation areas and important habitats for particularly vulnerable species could be prioritised for measures to minimise artificial lighting’s negative effects on biodiversity. This may be done by classifying protected natural environments into different zones and applying more constrained principles to limit lighting. The light pollution sensitivity of various environments and ecosystems suggests that different mitigation strategies and adaptations should be used depending on landscape characteristics, species sensitivity and other factors that may determine whether artificial light may be detrimental. Issues of the currently used measurement methods for artificial light at night are reviewed. We also propose and discuss the principles and benefits of using standardized measurement methods and appropriate instrumentation for field measurements of artificial light concerning the environmental impact of light pollution.