Sitagliptin is a dipeptidyl- peptidase inhibitor used to treat high blood sugar levels caused by type2 diabetes. Absorption of Sitagliptin is 87% orally bioavailable and taking it with or without food does not affect its pharmacokinetics. Sitagliptin reaches maximum plasma concentration in 2 hours. Now in this present analytical research world quality by design or design by expert technique is used to get improved method for method validation. This concise review work can guide an analyst to choose most appropriate method for a best analytical method development and validation. This assessment encompasses various analytical methods such as UV Spectrophotometry, High performance liquid chromatography [HPLC], Liquid chromatography – Mass spectrometry (LC-MS), High performance thin layer chromatography (HPTLC) and Ultra performance liquid chromatrography (UPLC) for the estimation of sitagliptin in single and/or in combination.