For a wound to successfully heal, four extremely involuntary automated overlapping stages must occur. These stages include hemostasis, the inflammatory phase, proliferation, and remodelling, The objective of the study was to determine efficacy of polyherbal formulation (i.e, Echinops echinatus, Tabernaemontana divaricata, and Portulaca quadrifida) extract against wound healing activity upon topical application. The collected polyherbal plants was extracted with ethanol. Results showed that The wound healing activity of ethanolic extract of selected polyherbal formulation was studied by using excision wound model and the extract showed significant wound healing activity when compared to control and similar to standard FSC (Framycetin sulphate cream). It can be concluded based on findings that polyherbal formulation extract may be used as an alternative medicine in the healing of wound. Keywords: Polyherbal formulation, Excision model, Wound healing, Diabetic Rats