The eCLIP data provided here is a subset of the eCLIP data of RBFOX2 from a study published by Nostrand et al. (2016, http://dx.doi.org/10.1038/nmeth.3810). The dataset contains the first biological replicate of RBFOX2 CLIP-seq and the input control experiment (*fastq files). The data was changed and downsampled to reduce data processing time, thus the datasets does not correspond to the original data pulled from Nostrand et al. (2016, http://dx.doi.org/10.1038/nmeth.3810). Also included is a text file (.txt) encompassing the chromosome sizes of hg19 and hg38 obtained from UCSC (http://hgdownload.cse.ucsc.edu/goldenPath/hg19/bigZips/hg19.chrom.sizes, http://hgdownload.cse.ucsc.edu/goldenPath/hg38/bigZips/hg38.chrom.sizes) and a genome annotation for hg19 (.gtf) taken from Ensembl (http://ftp.ensemblorg.ebi.ac.uk/pub/release-74/gtf/homo_sapiens/) and for hg38 taken from the Galaxy libraries (https://usegalaxy.eu/library/list#folders/F30cab321d898d2fb/datasets/9ba790aa79c9cf23). The data is used for a galaxy training course about CLIP-Seq data analysis.