Fig. 2. A phylogram based on the small subunit of 18S rRNA nuclear gene fragments for the 58 sequences obtained from the parasites isolated from different parts of the American mink digestive tract: s – stomach; d – duodenum; si – small intestine (bold – Aonchtheca putorii, navy blue and bold – Molineus patens) and sequences achieved from GeneBank (http://www.ncbi.nlm.nih. gov/) (in italics) of the other 8 Trichuridae species (phylum Nematoda). All newly achieved sequences are in bold (details in Appendix 1, Fig. S2). The evolutionary relationships of the taxa were implied using the maximum likelihood estimation method (Tamura and Nei, 1993) embedded in MEGA6 software (Tamura et al., 2013). The tree with the highest log likelihood (– 1262.9129) is shown. The evolutionary distances were computed using the maximum composite likelihood method (Tamura et al., 2004) and are expressed as a number of base substitutions per site. All positions containing gaps and missing data were eliminated. There were a total of 456 positions in the final dataset.

Published as part of Kołodziej-Sobocinska, Marta, Tokarska, Małgorzata, Zalewska, Hanna, Popiołek, Marcin & Zalewski, Andrzej, 2021, Digestive tract nematode infections in non-native invasive American mink with the first molecular identification of Molineus patens, pp. 48-52 in International Journal for Parasitology: Parasites and Wildlife 14 on page 50, DOI: 10.1016/j.ijppaw.2020.12.006, http://zenodo.org/record/13266936