Figs 9-12. Fig. 9, patterns of PCR-multiplex for the COI mitochondrial gene. Fig. 10, PCR-RFLP patterns of the COI gene with NlaIV enzymes. Fig. 11, patterns of PCR-multiplex for the RAG nuclear gene. Fig. 12, PCR-RFLP patterns of the RAG gene with the BsrI enzyme. The species are indicated as: column 1, Cichla kelberi Kullander & Ferreira, 2006; column 2, Cichla piquiti Kullander & Ferreira, 2006; column 3 and 4, Cichla kelberi; column 5 and 6, Cichla piquiti; column 7 and 8, carijó; M, 1 kb molecular weight marker.

Published as part of Mourão, Andrea A. F., Freitas-Souza, Diogo, Hashimoto, Diogo T., Ferreira, Daniela C., Prado, Fernanda D. do, Silveira, Rosicleire V., Foresti, Fausto & Porto-Foresti, Fábio, 2017, Molecular and morphological approaches for species delimitation and hybridization investigations of two Cichla species, pp. 1-9 in Iheringia, Série Zoologia (e2017016) 107 on page 6, DOI: 10.1590/1678-4766e2017016, http://zenodo.org/record/13264924