Raw Diffraction Images Of Chrimson

Chrimson is a red-shifted channelrhodopsin from the algae Chlamydomonas noctigama. The crystals were obtained within the lipidic cubic phase (LCP). 45 small-wedge (10 or 20°/crystal) and 8 helical (50-100°/crystal) datasets collected from loop-harvested microcrystals using EIGER X 9M detector at a wavelength of 1 Å on BL32XU, SPring-8. The crystals belonged to space group P212121 with unit cell parameters a=61.0, b=81.4, c=170.1 Å. 13 datasets were merged at 2.6 Å resolution in the published result (Nature Comm., 2018; PDB code: 5ZIH) using KAMO; see processing note https://github.com/keitaroyam/yamtbx/wiki/Processing-Chrimson-data-(5ZIH)

Related Organizations

University of Tokyo
Japan
RIKEN
Japan

Keywords

x-ray diffraction, bl32xu, spring-8, protein crystal, small wedge

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