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A Comparative Study On The Haemocytes Of The Fifth Instar Larvae Of A Bivoltine Breed And Multivoltine Breed Of The Mulberry Silkworm, Bombyx Mori L.

Authors: M. Vinotha*;

A Comparative Study On The Haemocytes Of The Fifth Instar Larvae Of A Bivoltine Breed And Multivoltine Breed Of The Mulberry Silkworm, Bombyx Mori L.

Abstract

{"references": ["Akai, H. and Sato, S., 1973. Ultrastructure of the larval haessmocytes of the silkworm, Bombyx mori L. (Lepidoptera: Bombycidae). J. Insect Morphol. Embryol., 2(3), 207-231.", "Arnold, J.W. and Hinks, C.F., 1976. Haemopoiesis in Lepidoptera. I. The multiplication of circulating haemocytes. Can. J .Zool., 54, 1003-1012.", "Balavenkatasubbaiah, M., Nataraju, B., Thiagrajan, V. and Datta, R.K., 2001. Haemocytes counts in different breeds of silkworm Bombyx mori L. and their changes during progressive infection of BmNPV. Indian J. Seric., 40,158-162.", "Begum, R., Gohain, R. and Hazarika, L.K., 1998. Detoxification of deltamethrin by the haemocytes of Philosamia ricini Boisd. (Lepidoptera: Saturniidae). Indian J. Sericul., 37(2), 142-147.", "Bora, D.S. and Handique, P., 2008. Effect of Catharanthus roseus on haemocytes of Antheraea assama. J. Environ. Biol., 29, 202-205.", "Brehelin, M., 1982. Comparative study of structure and fonction of blood cells from two Drosophila species. Cell Tissue Rex., 221, 607-615.", "Cantwell, G.E., 1973. Methods for determining the level of Nosema infection in honeybees. In \"Insect diseases\" (G. E. Cantwell, ed.). Marcel Dekker, Now York, pp. 539-542,", "Chain, B.M. and Anderson, R.S., 1982. Selective depletion of the plasmatocytes in Galleria mellonella following injection of bacteria. J. Insect Physiol.. 28. 377-384.", "Dunn, P.E. and Drake, D.R., 1983. Fate of bacteria injected into na\u00efve and immunized larvae of the tobacco hornwormManducasexta . J Invertebr. Pathol.,41, 77- 85.", "Eslin, P. and Prevost, G., 1998. Hemocyte load and immune resistance to Asobara tabida are correlated in species of the Drosophila melanogaster subgroup. J. Insect Physiol., 44, 807\u2013816.", "Rowley, A.F., 1977. The role of the haemocytes of Clitumnus extradentatus in haemolymph coagulation. Cell Tiss. Res. 182, 513-524.", "Saad, I., 2005. Biological and physiological studies on the silkworm. Ph.D. Thesis. Fac Agric Tanta University, Tanta, pp. 1-121.", "Salt, G., 1970. The cellular defense reactions of insects. Cambridge Monographs in Experimental Biology, Cambridge University Press, London and New York, No.16, p.188.", "Sato, S., Akai, H. and Swada, H., 1976. An ultrastructural study of capsule formation by Bombyx mori L. Annot. Zool. Jpn., 49, 177-188.", "Shapiro, M., 1968. Changes in the haemocyte population of the wax moth, Galleria mellonella, during wound healing. J. Insect Physiol., 14, 1725-1733.", "Strand, M.R. and Pech, L.L., 1995. Immunological basis for compatibility in parasitoid-host relationships. Ann. Rev. Entomol., 40, 31-56.", "Takeda, H., Kawakuchi, Y., Ohsika, T., Maekawa, H. and Tsuchida, K., 1996. Impaired yolk protein uptake by oocytas of a Bombyx mori mutant. Insect Biochem. Mol. Biol., 26, 607-616."]}

Haemocytes perform various physiological functions in the body of an insect. Total and specific haemocyte populations indicate the immunological status of an insect. The total number of haemocytes (THC) and the different haemocyte count (DHC) vary during the growth and metamorphosis of different breeds of the silkworm. The THC and DHC of a multivoltine breed were compared with that of a bivoltine breed in this work. THC of multivoltine breed ranged from 6.4 x 103/ mm3 on the 1st day to 10x103/mm3 on the 5th day. THC of bivoltine breed ranged from 3.2x 103/ mm3 on the 1st day to 6.4 x 103/mm3 on the 5th day. Only four different types of haemocytes were observed in the haemolymph of both breeds. They were prohaemocyte, granulocytes, spherule cells and oenocytoids. ʽtʼ test values indicate that significant differences were observed between the two breeds in terms of THC and DHC.

Keywords

Prohaemocyte, Granulocytes, Spherule cells, Oenocytoids., 10.5281/zenodo.1312696

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