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ZENODO
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License: CC BY
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ZENODO
Dataset . 2018
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Actinidia eriantha Accession EA01_01 low coverage genome assembly

Authors: Crowhurst, Ross; McCallum, John;

Actinidia eriantha Accession EA01_01 low coverage genome assembly

Abstract

Scaffolds file split to meet Zenodo upload limits.Paired-end small insert libraries of nuclear DNA of accession 'EA01-01' was sequenced on the Illumina HiSeq 2000 platform to circa 62x coverage. Reads were error-corrected with the Allpaths LG error corrector(Butler 2008) ,trimmed using Fastq-Mcf (https://github.com/ExpressionAnalysis/ea-utils) with parameters q=20 t=0.0001 and assembled using Soap Denovo2 (http://soap.genomics.org.cn/soapdenovo.html) with kmers 53/63/73. Following gap closure with GapCloser with two iterations, scaffolds were shredded , reassembled with Newbler (parameters -het -large -mi 90 -ml 40) and subjected to a reference-based scaffolding against draft assembly of Red5 (Pilkington 2018) with SSPACE (Boetzer 2010).

Draft assembly scaffolds of kiwifruit Actinidia eriantha 'EA01_01'. This is a female vine derived from seed collected on Qi-Yuan Mt., Min-Qing, Fukien on 15/11/75 by Li Lai-Yung, Professor of Subtropical Pomology, University of Fukien, Peoples Republic of China and provided to the New Zealand DSIR in 1975

{"references": ["Genomic Analysis of a Super-High Vitamin C Trait in Polyploid Hybrids of Kiwifruit (Actinidia spp.). John McCallum, Susan Thomson ,William Laing, Mareike Knaebel,Jibran Tahir, Simon Deroles, Sean Bulley, Ross Crowhurst\u00a0, Elena Hilario, Andrew Catanach, Alan Seal. (2018) Submitted"]}

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Keywords

assembly, kiwifruit, Actinidia, genomics, sequencing

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popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
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influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
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impulse
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