The present dataset contains chemical, characterization, and ecotoxicological data upon exposure of benzotriazole (BTA) in the soluble form and nanoforms (Mg-Al LDH-BTA and Zn-Al LDH-BTA). Chemical analyses data include the quantification of relevant anions (nitrites, nitrates, phosphates and chlorides, determined through High-Performance Liquid Chromatography) and target metals (Al, Mg and Zn) determined through Inductively Coupled Plasma Optical Emission Spectrometry). Characterization data include DLS (size) and zeta potential analysis. Ecotoxicological data includes acute and chronic endpoints determined in a wide-range of marine species.