The present study was carried out to screen the phytochemicals of the polyherbal plants (mixture of Andrographis paniculata, Andrographis alata, Adhatoda zeylanica, Gymnema sylvestre, Syzygium cumini and Justicia glabra) through preliminary phytochemical screening and GC-MS analyses. The powder of polyherbal plant mixture was extracted using ethylacetate as solvent. The preliminary phytochemical screening of the extract revealed the presence of bioactive compounds such as flavonoids, alkaloids, carbohydrate, saponin, phenols, triterpenoids, coumarins, emodins and fatty acid and then the extract was subjected to GC- MS analysis. The GC-MS analyses showed the presence of 12 bioactive compounds and these compounds are recommended to treat antidiabetic activity.